https://www.andrewwoolleylab.org/crosslinkers daily 0.75 2020-03-30 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585509262853-UBPUNBB026N60K1QZBNW/image-asset.png Crosslinkers IAC (iodoacetamide azobenzene) thiol-reactive reversible photoswitchable cross-linker, trans form stable in the dark, trans absorbs UV (λmax 370 nm;ε370=24000M-1cm-1) https://www.ncbi.nlm.nih.gov/pubmed/10760254 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585509262853-UBPUNBB026N60K1QZBNW/image-asset.png Crosslinkers IAC (iodoacetamide azobenzene) thiol-reactive reversible photoswitchable cross-linker, trans form stable in the dark, trans absorbs UV (λmax 370 nm;ε370=24000M-1cm-1) https://www.ncbi.nlm.nih.gov/pubmed/10760254 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585536593672-END9TGE7DGFO7MD01WXI/bsbca.png Crosslinkers bis-sulfonato-bis-chloroacetamido-azobenzene (BSBCA) water-soluble, thiol reactive cross-linker, UV, ε370=29000M-1cm-1 https://www.ncbi.nlm.nih.gov/pubmed/17406582 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585536951901-RWV6APFZIWEGLOA6NFSD/TMO.png Crosslinkers tetra-ortho-methoxyazobenzene-chloroaceamide (TOM) green/red, λmax=490nm, photoswitchable cross-linker https://www.ncbi.nlm.nih.gov/pubmed/22082305 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585599393210-L8PDDKT7TMR5C982LKD5/azonium.png Crosslinkers tetra-ortho-methoxy azonium (PIP) red-light photoswitch, ε600 = ~20,000 M-1cm-1 in pH7.0 buffer; trans azonium form apparent pKa of 7.2 https://www.ncbi.nlm.nih.gov/pubmed/24214131 https://www.andrewwoolleylab.org/pyp-chromophore daily 0.75 2020-03-30 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585605373604-JPCZEQ5OCIGFDNHYNYSB/pyp.png PYP chromophore p-coumaric acid, thiophenyl ester activated chromophore for PYP contact us or see: https://link.springer.com/protocol/10.1007/978-1-4939-3512-3_6 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585605373604-JPCZEQ5OCIGFDNHYNYSB/pyp.png PYP chromophore p-coumaric acid, thiophenyl ester activated chromophore for PYP contact us or see: https://link.springer.com/protocol/10.1007/978-1-4939-3512-3_6 https://www.andrewwoolleylab.org/home daily 1.0 2025-03-01 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/54831e74-5c15-4b51-9fb0-2169a1c54fd6/2024-06-28+Woolley+Group.jpg Home - The group The Woolley Lab, seen here hosting the 2024 Protein Engineering Canada Conference. https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/a4c0c1b8-e1de-4633-b0c6-0821ed7fc024/Sheep.png Home - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.andrewwoolleylab.org/photopharmacology daily 0.75 2020-03-30 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585071680604-82QFLY2W3710G0IVVBK1/helix.png Photopharmacology Trans-to-cis isomerization of an azobenzene cross-linker drives unfolding of an helical peptide. https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585011199230-UNJZMET7Y01F089K90IV/Woolley_ISOP_2016.jpg Photopharmacology Careful choice of X and Y allows photoswitching with red light, which can penetrate tissue. https://www.andrewwoolleylab.org/protein-engineering daily 0.75 2024-08-22 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585081170303-U3VNQA83WJWA6IM89Q44/pypb6.jpg Protein Engineering BoPD (ribbons) bound to the dark state of PYP https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/bf54a7ac-e74f-43a4-94a6-d9f22b80048b/green+bicycl+image.png Protein Engineering - Make it stand out Cyanobacteriochromes respond to a wide range of wavelengths of light. https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585168340992-BLKE349C6L3A92K0MIYJ/sb-2018-00386r_0010.gif Protein Engineering A LOV-4EBP hybrid for controlling translation initiation https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585101334269-IVR1DFVA4O2JA5DQ2I0N/image%281%29.png Protein Engineering Green, orange, red and far-red tools built from cyanobacteriochromes https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/dfce4bcc-cb63-4ee2-9fa5-20b0d6ee8125/pyp+dark+vs+light.png Protein Engineering PYP changes conformation upon blue light irradiation, allowing the design of tools like BoPD. https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585168371544-TLRN4ROV5VLB8H1OIX26/nihms-735435-f0001.jpg Protein Engineering In the dark opto-DN-CREB (cyan) is inactive and CREB (green) binds DNA; Under blue light opto-DN-CREB (white) binds CREB and sequesters it. https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585081945700-8O5FCZ20VYSVSCPFA5NE/Denver_2020.jpg Protein Engineering Duplicating a beta strand in PYP creates a light switchable metamorphic protein. https://www.andrewwoolleylab.org/publications daily 0.75 2025-12-08 https://www.andrewwoolleylab.org/people daily 0.75 2025-12-19 https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/1585606422532-D9URPM0ZJ7ETZ0WLV0O6/drew.png People - Prof. Andrew Woolley Send an email: andrew.woolley@utoronto.ca https://images.squarespace-cdn.com/content/v1/5e56f2888d873019db825bcd/6cd14d5c-7418-4065-a84e-ba94f1951d99/Woolley_Dec2025_Potluck.jpg People December 2025 Potluck